Figure 2

Immunocytochemistry analysis of HEK293T cells expressing RAI3-GFP. Transiently transfected HEK293T cells expressing RAI3-GFP were subjected to immunocytochemistry analysis. The upper panels (anti-RAI3) show transfected cells incubated with anti-RAI3 antibody and subsequently with anti mouse AlexaFluor 546-labelled secondary antibody (A546). The immunostaning is pseudo-coloured in red. Co-localisation of the RAI3-GFP and A546 signals is shown in yellow in the merge panel. For the transfected control cells (Control panels) primary anti-RAI3 antibody was omitted in order to exclude unspecific binding of the A546 secondary antibody. Nuclei were stained using Draq5 and are pseudo-coloured in blue. Imaging was carried out by confocal microscopy using a 40× air objective. Scale bar: 20 μm