Figure 6
From: KIF11 inhibition for glioblastoma treatment: reason to hope or a struggle with the brain?
Effect of the Ispinesib analog 1 on induction of apoptosis in U87MG. The cells after cell adhesion were incubated for 24 hours with compound 1 in a concentration range from 10 nM to 30 μM. The level of caspase 3 activation has been used as an indicator of apoptosis induction. The caspase 3 activation level was assessed after 24 hours of cell treatment with the compound at increasing concentrations (from 10 nM to 30 μM). The Ispinesib analog 1 induced a 2-fold increase of caspase 3 levels, suggesting that this compound was a strong inducer of apoptosis in GBM cells.