Table 1 Primers used to clone microRNA precursors in this study
From: Systematic validation of predicted microRNAs for cyclin D1
MicroRNA | 5' primer | 3' primer |
|---|---|---|
miR-19a | GCATCTACTGCAGTGAAGGC | GCGGCCGCAGTTTGATTGGGCGACAGG |
miR-491 | AGATGGCTTTCTGGGTAGCC | GCGGCCGCTAACAGTAACACGTATGGGC |
miR-296 | CCTCCTTGGAGCTGAGATGG | GCGGCCGCGTGGGAGGTAGGTAAAACTC |
miR-532 | CATCTGTTCTCGTATGTGTG | GCGGCCGCACGTAAGAGAGGGTTTACC |
miR-188 | CTTCCCTCTCCAGTGCATAG | GCGGCCGCTCCTGCAGGATCCATGTAAG |
miR-579 | CAGTGACAGAGTTGTGTAAC | GCGGCCGCGTGGAAACAAGTTGCATGTC |
miR-501 | AGACCCTCAAGTGTGATCTG | GCGGCCGCAGTATATGCAGGGGCAATG |
miR-548c | TCAGGGTTTCACCATGTTGG | GCGGCCGCACAGAGCAAGACTCCATCTC |
miR-219-1 | CGTTTACTCTAGAGTCCTCG | GCGGCCGCACAGAGGGCTCGTGAAAAGG |
miR-340 | TGGCTACCTGTTCAGCTTTC | GCGGCCGCAGCAGTTTACCAGAATACCG |
miR-519b | CTGGACACAAAACTCAGGAG | GCGGCCGCGTCTGGGTCACAGAGCAAG |
miR-155 | TGCCTAAAGGTAACAATGTC | GCGGCCGCGGTTGAACATCCCAGTGAC |
miR-519e | GGTATGTGTGGTAGGCTTTG | GCGGCCGCAACTAGCCTAAGAGGTCTAG |
miR-515-1 | TACATGCCACCACAGGCGGC | GCGGCCGCCTGGGCCACAGAGAGAGAC |
miR-139 | GATCGTGCAGGCAACTCTTG | GCGGCCGCTGGATAACAAGGCAGTATGG |
miR-494 | TTCCCGGGCAACCTCTTTTC | GCGGCCGCTCCTTCAACCACAGAAGCAC |
miR-203 | CGTCTAAGGCGTCCGGTACG | GCGGCCGCTTCCCACAGCACAGCCCGGC |
miR-142 | GATGGGGTGGAGCCTTTAGG | GCGGCCGCAACCAGGAAGGGCAGGAAAG |
miR-450-1 | TGATTATCTCTGAGTTGTGG | GCGGCCGCAAAATGATCCCAATACAC |
miR-193a | AGTTTCTCGGCGCATAACTC | GCGGCCGCTATTTCTCCAGCGAAGTG |
miR-193b | GTTGTCCGGGAAGCTTTTAG | GCGGCCGCCCTCGAAGGGACCTTTTAG |
miR-487b | TGGTCTGGGTCCCTGCTTCC | GCGGCCGCAGTCATAAAACCACCACCAC |
miR-487a | CAGTCTCATGAGGGAGTGCC | GCGGCCGCTCTCACTGGTCAACTTCCC |
miR-301 | AGGGCTTGTTTTACATGGCG | GCGGCCGCCAGACGTGTTTCATAATGC |
miR-130a | CTGCTTTAGTGGGTCCTGTC | GCGGCCGCTAACGGAGGCAGTGTCTATC |
miR-130b | GTCAAAGCATCTGGGACCAG | GCGGCCGCACCTGATCCTCTGATGGAG |
miR-148a | CTTTGCTGTGACATTGCGAC | GCGGCCGCTTTGGGGGGATTTTGTTCCC |
miR-503 | CTCGTGGGGAAGGTAGAAGG | GCGGCCGCAAGATAGGTTTTCTTCGTTG |
miR-152 | AGCAGCCAACTCAGAACTCG | GCGGCCGCAAGGTCCACAGCTGGTTCTG |
miR-95 | CCTCCTGAGGAATCCAGGCG | GCGGCCGCGATCGGGCCCTTGCGAAGCC |
miR-550 | GAGCCTTCTAACCACAACCC | GCGGCCGCATAAAGCAGTTCCTACACTC |
miR-545 | TCATGCCAGTGTCTAGTTCC | GCGGCCGCCACCAAGAAAGCAAGTGTC |
miR-424 | ACCCACTACGTTGTTCCAAG | GCGGCCGCAGAGCACCGATCGCTCACTG |
miR-15a | AAAGGTGTACTGCAAGGAAC | GCGGCCGCACTGCTGACATTGCTATC |
miR-16 | GCTAGGTTGGATGAATCCTAC | GCGGCCGCCATCCAAAGTGTTACAAAG |
miR-497 | CCTGAGCTGAGTTCCTACAG | GCGGCCGCGTGTGGGCAACAAAGACTCC |
miR-195 | GTGACACAAAAAACATCTGG | GCGGCCGCATAAGCATCTGCCCCCAACC |
miR-515-2 | CGAGTAGCTGGGACTACAGG | GCGGCCGCATACCAGCTACTCGGGAGGC |
miR-144 | ATTACGCCATCTCTGGCTTG | GCGGCCGCACGTTTTCTGGGTATAGC |
miR-34a | ATGGAGTCTTGCTAGTTGCC | GCGGCCGCTACGTGCAAACTTCTCCCAG |
miR-449a | TTGAAGTAATACATTTTGTG | GCGGCCGCACTGTCTACTCCGAAAGAC |
miR-146a | GTAGAGACAAATTCTCCATG | GCGGCCGCTTTCTCACAGGAACTCACAC |
miR-655 | AATCCGTGGAGGAAGCTTTC | GCGGCCGCACAGCAAACACAGCAAAGC |
miR-374 | GTTCCTTTATTGGAAGTCTG | GCGGCCGCAGGCACACAATAAATGTTTG |
miR-524 | AGTAGAGACAGGGTTTCACC | GCGGCCGCGATAGAGAGACTCTGTCCGC |
miR-520d | CTGGTGTCTAACTCCTGAGA | GCGGCCGCTGACCATTTGAAGCCAAGAG |
miR-376a-1 | CCTGAGTAGGTGCAAAGATG | GCGGCCGCATTATGTGTGCACCAAGGGC |
miR-23b | AATTGTGTGTGTCGCATGGC | GCGGCCGCGGTGCAGAACTTAGCCACTG |
miR-23a | GCCATGCAAGTTGCTGTAGC | GCGGCCGCTTCCTGCTGAACTGAGCCAG |
miR-199a-2 | AGCGCATACCCAAACCTCCC | GCGGCCGCGTCAAGAAGGCCTTACCCGC |
miR-199b | GGGGTTGGACACTAGGTAGG | GCGGCCGCAGGTTTTGGGGTGAGGGAGG |
miR-194-1 | CTAATGGCATTATCTCACAG | GCGGCCGCGATGACTTTAATCCTCTTCC |
miR-202 | CCTGCGCATAAATGTGGAGC | GCGGCCGCTCCTCTCTGCATCCTGGGTC |
miR-875 | TATACCTCAGTTTTATCAGGTG | GCGGCCGCGTGCATAGCTTCTGTAAAGG |
miR-511-1 | AATGGCCTCTAGTTGCATCC | GCGGCCGCATAGATCACTTACCTGAGGG |