Effect of anti-miR-503 and miR-503 binding sites on the Luc-CCND1-3'-UTR activity. 293T cells were transfected with Luc-CCND1-3'-UTR-2 along with either scrambled oligo or anti-miR-503 and the harvested for luciferase assays 24 h after transfection. To determine the importance of miR-503 binding sites, the same cells were transfected with Luc-CCND1-3'-UTR-2-d, in which the miR-503 binding sites were deleted. Along with the reporter Luc-CCND1-3'-UTR-2-d was either vector or miR-503. Values are means ± SE of three separate experiments. **, p < 0.01; n.s., not significant.