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Table 1 Primer sequences, amplification sizes and annealing temperatures used in RT-PCR.

From: Cytoplasmic Kaiso is associated with poor prognosis in non-small cell lung cancer

Primer sequence(5' -> 3')

Amplification range

PCR setting Annealing Number of cycles

Kaiso

TGCCTATTATAACAGAGTCTTT

719–966 (NM_006777.3)

50°C, 40 sec 30 cycles

 

AGTAGGTGTGATATTTGTTAAAG

  

Matrilysin

TCTTTGGCCTACCTATAACTGG

241–660

(NM_002423.3)

53°C, 40 sec 35 cycles

 

CTAGACTGCTACCATCCGTC

  

β-Actin

AGAGCTACGAGCTGCCTGAC

797–1096

(NM_001101.3)

55°C, 40 sec 30 cycles

 

AGTACTTGCGCTCAGGAGGA

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