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Figure 6 | BMC Cancer

Figure 6

From: Cytoplasmic Kaiso is associated with poor prognosis in non-small cell lung cancer

Figure 6

Down-regulated nuclear Kaiso increases matrilysin transcription in three kinds of lung cancer cell lines. (A) The electrophoresis images of matrilysin mRNA in BE1, BE1+empty, BE1+shRNA-Kaiso, BE1+IgG, and BE1+Kaiso antibody cells after RT-PCR. β-actin served as an internal control. The relative expression of matrilysin mRNA was significantly enhanced in BE1+shRNA-Kaiso and BE1+Kaiso antibody cells. Columns, mean (n = 3); bar, SD. *, p < 0.01. (B) The electrophoresis images of matrilysin mRNA in LTEP-A-2, LTEP-A-2+empty, LTEP-A-2+shRNA-Kaiso, LTEP-A-2+IgG, and LTEP-A-2+Kaiso antibody cells after RT-PCR. β-actin served as an internal control. The relative expression of matrilysin mRNA was significantly enhanced in LTEP-A-2+shRNA-Kaiso and LTEP-A-2+Kaiso antibody cells. Columns, mean (n = 3); bar, SD. *, p < 0.01. (C) The electrophoresis images of matrilysin mRNA in SPC-A-1, SPC-A-1+empty, SPC-A-1+shRNA-Kaiso, SPC-A-1+IgG, and SPC-A-1+Kaiso antibody cells after RT-PCR. β-actin served as an internal control. The relative expression of matrilysin mRNA was significantly enhanced in SPC-A-1+shRNA-Kaiso and SPC-A-1+Kaiso antibody cells. Columns, mean (n = 3); bar, SD. *, p < 0.01.

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