Figure 4

shRNA-Kaiso efficiently down-regulates nuclear Kaiso expression in three lung cancer cell lines. shRNA-Kaiso efficiently down-regulated nuclear Kaiso expression in three lung cancer cell lines. Specifically, nuclear staining of Kaiso was detected by immunofluorescence in BE1, LTEP-A-2, and SPC-A-1 cells (top rows in A, B, and C). After transfected with shRNA-Kaiso, BE1, LTEP-A-2, and SPC-A-1 cells showed significantly reduced green/yellow signals in the nucleus, while there was no signal detected in several transfected cells (bottom rows in A, B, and C), compared with controls (middle rows in A, B, and C). Results from RT-PCR and immunoblotting assays were shown in D and E. Little bands or dots can be detected after transfection with shRNA-Kaiso, which demonstrated that levels of Kaiso mRNA and protein were down-regulated significantly (p < 0.05). β-actin served as an internal control. Data were expressed as mean ± standard deviation (S.D.). Columns, mean (n = 3); error bars, S.D.