Kaiso expression in NSCLC by Western blotting. (A) Representative results of Kaiso protein expression in matched tumourous (T) and surrounding non-tumorous (N) tissues from 8 of 20 NSCLC patients. Lane T: tumor tissue; Lane S: Surrounding normal lung tissue. Samples: case 4; case 6; case 8; case 18. Band intensities indicate significant Kaiso up-regulation in tumorous in comparison with the non-tumorous tissue of the same patient. β-actin was used as a loading control to assure equal amounts of protein in all lines. (B) The ratio between the optical density of Kaiso and β-actin of the same patient was calculated and expressed graphically. The significant difference of Kaiso expression between tumorous (T) and non-tumorous (N) tissues was analyzed statistically. Kaiso immunoreactivity is greater in neoplastic tissues (p = 0.000). Data were expressed as mean ± standard deviation (S.D.). Columns, mean (n = 20); error bars, S.D.