Immunohistochemical analysis of Kaiso expression. Kaiso was expressed in the cytoplasm of normal adult bronchial epithelial cells (A) and glands (B). The expression of Kaiso was increased in metastases of lung adenocarcinomas (D) and squamous cell carcinomas (F), compared to the matched primary tumor (C, D). Nuclear Kaiso staining was observed occasionally primary tumors (G, black arrow), with cytoplasmic staining of tumor cells in paired metastases. A magnification scale bar of 20 μm is shown.