Correlation of HDGF copy number and expression by microarray analysis and validation of HDGF expression using RT-PCR. A) HDGF copy number ratio and B) HDGF expression ratio by microarray analysis in ESFT patient samples (n = 16). The patients in figure A) and B) are in the same order, and labelled according to the codes of the DNA samples. C) Correlation of HDGF copy number and expression ratio (correlation 0.844, P < 0.001, q-value 0.024). D) HDGF expression in ESFT patient samples (n = 42) by RT-PCR analysis, on y-axis refers to log of fold-change in HDGF gene expression and x-axis to patients RNA code number. Kaplan-Meier plots of ESFT patient (n = 42) survival according to D) event-free survival and E) overall survival, patients with high expression of HDGF in bold line and patients with low expression in hatched line.