Figure 1

A BrdU incorporation assay indicated that rhChM1 directly suppressed DNA synthesis of HUVECs (A) and HepG2 cells (C) cultured on plates. rhChM1 significantly inhibited DNA synthesis in HUVECs, with or without FGF-2 (A), but did not in NHDFs with FGF-2 (B). HepG2 and HeLa cells were cultured in growth medium for 36 hrs, and then in fresh growth medium containing rhChM1 for the next period as indicated. rhChM1 inhibited DNA synthesis of HepG2 in a dose-dependent manner (C), but had no effect on HeLa cells (D). Each column and bar represent the mean and SD (n = 4). *: p < 0.05; **: p < 0.001; NS: not significant.