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Table 1 Details of immunohistochemistry procedure

From: Expression of miR-21 and its targets (PTEN, PDCD4, TM1) in flat epithelial atypia of the breast in relation to ductal carcinoma in situ and invasive carcinoma

Antibody

clone (source)

dilution

Antigen retrieval

PTEN

6H2.1 (Cascade BioScience)

1:100

Microwave (10 mM sodium citrate pH = 6.0)

PDCD4

ab51495 (Abcam)

1:100

Microwave (10 mM sodium citrate buffer pH = 6.0)

TM1 (alpha)

ab55915 (Abcam)

1:200

Protease 0.1%, 30 min