Skip to main content
Figure 3 | BMC Cancer

Figure 3

From: Groucho binds two conserved regions of LEF-1 for HDAC-dependent repression

Figure 3

Groucho and AES repress LEF-1/β-catenin mediated activation of transcription. Luciferase activity of a transiently transfected TOPFlash reporter assay using COS-1 cells. The TOPFlash (100 ng) reporter with three multimerized LEF/TCF DNA consensus sequences in front of the c-fos minimal promoter was activated 5 and 3-fold by LEF-1•β-catenin complexes (200 and 400 ng of expression plasmid respectively). This level of activation was set equal to one for comparison to the amount of TOPflash activity measured in the presence of Groucho or AES expression vector (250 and 500 ng as shown). Increasing amounts of Groucho or AES expression vector cause increasing repression of reporter activity. All data was normalized with a co-transfected CMV β-galactosidase reporter. Error bars represent the standard deviation among three experiments. Student t test was performed and p values are represented by asterisks. One asterisk represents a value < 0.05 and two asterisks represent a value of < 0.01. Western analysis of Groucho, AES and TLE-2 expression shows a dose-dependent increase in protein expression (insets).

Back to article page