Figure 1

Schematic representation of the LEF-1 and LEF-1 deletion constructs, Groucho and APC. A) A LEF-1 protein schematic highlights four features: the β-catenin binding domain (βBD; aa1-67), the context dependent regulatory domain (CRD; aa67-296), an alternative exon in the CRD (black bar; aa214-241) and the high mobility group DNA binding domain (HMG; aa296-384). LEF-1 deletions tested in a GST pulldown assay are also depicted. B) General domain structure of Groucho/TLE proteins and truncated AES isoforms. The Q domain (aa1-132) is required for tetramerization and the GP domain interacts with HDACs. Three additional domains of protein-protein interaction are shown: the CcN, SP and WD-repeat domains. C) Schematic of Adenomatous polyposis coli (APC) and APC mutations found in Familial Adenomatous Polyposis (FAP) cancer cell of type I (DLD-1, SW480, and Colo 320) and type II (HT-29) and Hereditary Non-Polyposis Colon Cancer (HNPCC) colon cancer cell lines (LS174T and HCT116) [29, 34]. Asterisks indicate nuclear export signals and diamonds indicate nuclear localization signals. The MCR (mutation cluster region) refers to the most common site of truncating mutations found in colon cancer. This region overlaps a region comprised of 15 and 20 amino acid repeats that bind beta-catenin. [28, 48].