Figure 3

Effects of MP470, Erlotinib, and MP470-Erlotinib treatment on Akt activity. (a). LNCaP cells were treated with DMSO or different doses of Erlotinib, MP470, IM or combinations as indicated for 30 hr. Phospho(Ser473)-Akt and total Akt were detected by immunoblotting. β-actin antibody was used as the loading control. (b). LNCaP cells were grown in androgen-depleted medium, phenol red-free RPMI 1640 supplemented with 10% charcoal/dextran-treated FBS for 10 days. The cells were treated with 10 μM of Erlotinib, MP470, IM alone or Erlotinib plus MP470 and Erlotinib plus IM for 24 hr, and Akt phosphorylation was analyzed by Western blotting.