Metastatic behaviour of primary human tumours in a zebrafish xenotransplantation model

Marques, Ines J; Weiss, Frank Ulrich; Vlecken, Danielle H; Nitsche, Claudia; Bakkers, Jeroen; Lagendijk, Anne K; Partecke, Lars Ivo; Heidecke, Claus-Dieter; Lerch, Markus M; Bagowski, Christoph P

doi:10.1186/1471-2407-9-128

BMC Cancer

Table 1 Tumour xenotransplantations into 2 day old zebrafish

From: Metastatic behaviour of primary human tumours in a zebrafish xenotransplantation model

	Tumour Transplants (grade and pTMN stage)	Number transplants (survivors)	Migration [presence of micro-metastases at 3 dpi]			total >5 [%]	Control Transplants	Number transplants (survivors)	Migration [presence of micro-metastases at 3 dpi]			total >5 [%]	p-value
			<5	5 to 20	>20				<5	5 to 20	>20
1	Pancreas G2; T3; N1 (13/14) R1, M1	80 (64)	0	20 [4]	25 [20]	70.3%	Normal Pancreas	80 (73)	0	0	0	0	6.3 × 10^-5
2	Pancreas G2; pT3, pN1, pM1	60 (42)	2 [0]	18 [4]	6 [4]	57.1%	Chronic Pancreatitis	60 (50)	0	0	0	0	1.1 × 10^-5
3	Pancreas G2; pT1, pN1 (1/18) pM1	80 (46)	0	18 [5]	8 [7]	56.5%	Chronic Pancreatitis	80 (59)	0	0	0	0	1.9 × 10^-4
4	Pancreas G3; pT3, pN1 mi (1/11) pM1	80 (65)	0	24 [6]	12 [9]	55.4%	Chronic Pancreatitis	80 (58)	0	0	0	0	5.4 × 10^-5
5	Colon G2; pT3 pN1 (2/14) LN metas.	80 (66)	0	15 [7]	14 [10]	43.9%	Normal Tissue	80 (72)	0	0	0	0	0.9 × 10^-6
6	Stomach G2: pT3 pN2 (11/18 LK)	80 (62)	0	23 [6]	11 [9]	54.8%	Normal Tissue	80 (68)	0	0	0	0	2.9 × 10^-5
7	Stomach G2; ypT2b, ypN0 (0/20)	80 (48)	0	18 [5]	7 [6]	52.3%	Normal Tissue	80 (51)	0	0	0	0	1 × 10^-6
	Primary tumour cell injections	Number transplants (survivors)	Migration [presence of micro-metastases at 3 dpi]			total >5 [%]	Primary control cell injections	Number transplants (survivors)	Migration [presence of micro-metastases at 3 dpi]			total >5 [%]	p-value
			<5	5 to 20	>20				<5	5 to 20	>20
1	Pancreas	80 (63)	3 [0]	38 [10]	4 [4]	66.6%	Normal Pancreas	80 (69)	0	0	0	0	9.1 × 10^-5
2	Pancreas	80 (66)	0	23 [7]	3 [2]	39.4%	Chronic Pancreatitis	80 72	2 [0]	0	0	0	7.6 × 10^-5
3	Pancreas	80 (63)	0	22 [7]	6 [5]	44.4%	Chronic Pancreatitis	80 (61)	1 [0]	0	0	0	4 × 10^-4
4	Pancreas	80 (63)	0	23 [6]	5 [4]	44.4%	Chronic Pancreatitis	80 (63)	1 [0]	0	0	0	5 × 10^-4
5	Colon	80 (65)	0	24 [7]	5 [4]	44.4	Normal Tissue	80 (59)	1	0	0	0	2.7 × 10^-5
6	Stomach	80 (68)	0	24 [6]	0	35.3	Normal Tissue	80 (69)	0	0	0	0	6.8 × 10^-6

Tumours as numbered in the table: ¹Pancreas head carcinoma; ²Well differentiated ductal adenocarcinoma of the Pancreas head; ³Moderately differentiated ductal adenocarcinoma of the Pancreas head; ⁴Poorly differentiated adenocarcinoma of the ampulla vateri with infiltration of the pancreas; ⁵Adenocarcinoma of the colon; ⁶Moderately differentiated adenocarcinoma of the stomach; ⁷Moderately differentiated tubular adenocarcinoma of the stomach. For the tumour cell injections in the lower half of the table numbers 1–6 indicate the tumour sources of the dissociated cells. There is a significant difference between all tumours and controls and all p-values of a student t-test were below 0.001. Tumour grading and classification is according to histopathological examination. Development of micrometastases was assessed by the presence of daughter cells at 3 days post transplantation (dpt).

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ISSN: 1471-2407

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