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Table 1 Tumour xenotransplantations into 2 day old zebrafish

From: Metastatic behaviour of primary human tumours in a zebrafish xenotransplantation model

  Tumour Transplants (grade and pTMN stage) Number transplants
(survivors)
Migration [presence of micro-metastases at 3 dpi] total >5 [%] Control Transplants Number transplants
(survivors)
Migration
[presence of micro-metastases at 3 dpi]
total >5 [%] p-value
    <5 5 to 20 >20     <5 5 to 20 >20   
1 Pancreas
G2; T3; N1 (13/14) R1, M1
80 (64) 0 20
[4]
25 [20] 70.3% Normal Pancreas 80 (73) 0 0 0 0 6.3 × 10-5
2 Pancreas
G2; pT3, pN1, pM1
60 (42) 2
[0]
18
[4]
6
[4]
57.1% Chronic Pancreatitis 60 (50) 0 0 0 0 1.1 × 10-5
3 Pancreas
G2; pT1, pN1 (1/18) pM1
80 (46) 0 18
[5]
8
[7]
56.5% Chronic Pancreatitis 80 (59) 0 0 0 0 1.9 × 10-4
4 Pancreas
G3; pT3, pN1 mi (1/11) pM1
80 (65) 0 24
[6]
12
[9]
55.4% Chronic Pancreatitis 80 (58) 0 0 0 0 5.4 × 10-5
5 Colon
G2; pT3 pN1 (2/14) LN metas.
80 (66) 0 15
[7]
14
[10]
43.9% Normal Tissue 80 (72) 0 0 0 0 0.9 × 10-6
6 Stomach
G2: pT3 pN2 (11/18 LK)
80 (62) 0 23
[6]
11
[9]
54.8% Normal Tissue 80 (68) 0 0 0 0 2.9 × 10-5
7 Stomach
G2; ypT2b, ypN0 (0/20)
80 (48) 0 18
[5]
7
[6]
52.3% Normal Tissue 80 (51) 0 0 0 0 1 × 10-6
  Primary tumour cell injections Number
transplants
(survivors)
Migration
[presence of micro-metastases at 3 dpi]
total >5 [%] Primary control cell injections Number
transplants
(survivors)
Migration
[presence of micro-metastases at 3 dpi]
total >5 [%] p-value
    <5 5 to 20 >20     <5 5 to 20 >20   
1 Pancreas 80 (63) 3
[0]
38
[10]
4
[4]
66.6% Normal Pancreas 80 (69) 0 0 0 0 9.1 × 10-5
2 Pancreas 80 (66) 0 23
[7]
3
[2]
39.4% Chronic Pancreatitis 80 72 2
[0]
0 0 0 7.6 × 10-5
3 Pancreas 80 (63) 0 22
[7]
6
[5]
44.4% Chronic Pancreatitis 80 (61) 1
[0]
0 0 0 4 × 10-4
4 Pancreas 80 (63) 0 23
[6]
5
[4]
44.4% Chronic Pancreatitis 80 (63) 1
[0]
0 0 0 5 × 10-4
5 Colon 80 (65) 0 24
[7]
5
[4]
44.4 Normal Tissue 80 (59) 1 0 0 0 2.7 × 10-5
6 Stomach 80 (68) 0 24
[6]
0 35.3 Normal Tissue 80 (69) 0 0 0 0 6.8 × 10-6
  1. Tumours as numbered in the table: 1Pancreas head carcinoma; 2Well differentiated ductal adenocarcinoma of the Pancreas head; 3Moderately differentiated ductal adenocarcinoma of the Pancreas head; 4Poorly differentiated adenocarcinoma of the ampulla vateri with infiltration of the pancreas; 5Adenocarcinoma of the colon; 6Moderately differentiated adenocarcinoma of the stomach; 7Moderately differentiated tubular adenocarcinoma of the stomach. For the tumour cell injections in the lower half of the table numbers 1–6 indicate the tumour sources of the dissociated cells. There is a significant difference between all tumours and controls and all p-values of a student t-test were below 0.001. Tumour grading and classification is according to histopathological examination. Development of micrometastases was assessed by the presence of daughter cells at 3 days post transplantation (dpt).