Figure 1

Construction of recombinant Plasmid PB [Act-RFP, HSV-tk]. (A) PB [Act-RFP, HSV-tk] constructs. The HSV-tk gene was cloned in the BamHI-SpeI sites of the PB [Act-RFP] DS (pPB), generating PB [Act-RFP, HSV-tk] (pPB/TK), which consists of both HSV-tk and mRFP1 expression cassettes. PBL and PBR was a pair of PB repeat termini; (B) PB transposase helper constructs. The piggyBac transposase gene (PBase) driven by β-actin (Act) promoters was followed by rabbit β-globin polyA (rBG pA) (adapted from Ref [15]); (C) PCR analysis of pPB/TK: Lane 1: Marker; Lane 2: pPB/TK: PCR product of tk fragment; Lane 3: pPB/TK: PCR product of mRFP1 fragment; Lane 4: pPB: PCR product of mRFP1 fragment; Lane 5: pORF-HSVtk: PCR product of tk fragment; (D) Restriction analysis of pPB/TK: Lane 1: Marker; Lane 2: pPB/TK; Lane 3: pPB; Lane 4: pORF-HSVtk.