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Table 5 T50, Tto, Ttd and Ttd – Tto values for MGMT in FFPE CRC tissues

From: Quantitation of DNA methylation by melt curve analysis

Sample T50 Tto Ttd Ttd – Tto
U ref 74.17 ± 0.04 73.64 ± 0.04 75.54 ± 0.04 1.90 ± 0.03
M ref 78.70 ± 0.25 78.06 ± 0.37 80.43 ± 0.15 2.36 ± 0.25
No 74.18 ± 0.09 73.54 ± 0.06 77.24 ± 1.01 3.71 ± 0.95
Clonal 75.84 ± 0.21 73.62 ± 0.17 81.12 ± 0.37 7.50 ± 0.31
Complete 76.17 ± 0.07 73.70 ± 0.09 80.32 ± 0.45 6.62 ± 0.40
  1. U ref – unmethylated reference.
  2. M ref – methylated reference.
  3. No, Clonal and Complete – whole sections of FFPE CRC tissue with either no, clonal or complete loss of MGMT protein expression in tumor cells, as determined by immunohistochemistry.
  4. All values are the mean ± standard deviation of 6 replicate reactions.