Quantitation of DNA methylation by melt curve analysis

BMC Cancer

Table 5 T50, Tto, Ttd and Ttd – Tto values for MGMT in FFPE CRC tissues

Sample	T50	Tto	Ttd	Ttd – Tto
U ref	74.17 ± 0.04	73.64 ± 0.04	75.54 ± 0.04	1.90 ± 0.03
M ref	78.70 ± 0.25	78.06 ± 0.37	80.43 ± 0.15	2.36 ± 0.25
No	74.18 ± 0.09	73.54 ± 0.06	77.24 ± 1.01	3.71 ± 0.95
Clonal	75.84 ± 0.21	73.62 ± 0.17	81.12 ± 0.37	7.50 ± 0.31
Complete	76.17 ± 0.07	73.70 ± 0.09	80.32 ± 0.45	6.62 ± 0.40

U ref – unmethylated reference.
M ref – methylated reference.
No, Clonal and Complete – whole sections of FFPE CRC tissue with either no, clonal or complete loss of MGMT protein expression in tumor cells, as determined by immunohistochemistry.
All values are the mean ± standard deviation of 6 replicate reactions.

ISSN: 1471-2407