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Table 4 T50, Tto, Ttd and Ttd – Tto values for TIMP3 in OE33 cells treated with vehicle or 5-aza-2'-deoxycytidine

From: Quantitation of DNA methylation by melt curve analysis

Sample T50 Tto Ttd Ttd – Tto
U ref 75.52 ± 0.07 74.74 ± 0.16 77.36 ± 0.08 2.62 ± 0.24
M ref 78.07 ± 0.23 77.26 ± 0.37 79.83 ± 0.02 2.57 ± 0.35
veh 48 h 76.87 ± 0.18 75.63 ± 0.29 79.37 ± 0.04 3.74 ± 0.29
veh 72 h 76.80 ± 0.11 75.56 ± 0.17 79.33 ± 0.07 3.77 ± 0.19
veh 120 h 76.89 ± 0.18 75.70 ± 0.33 79.34 ± 0.08 3.65 ± 0.36
aza 48 h 75.40 ± 0.66 73.84 ± 1.15 78.90 ± 0.09 5.05 ± 1.12
aza 72 h 75.54 ± 0.42 74.34 ± 0.75 78.69 ± 0.06 4.35 ± 0.74
aza 120 h 75.49 ± 0.33 74.38 ± 0.57 78.45 ± 0.19 4.07 ± 0.64
  1. U ref – unmethylated reference.
  2. M ref – methylated reference.
  3. veh – OE33 esophageal adenocarcinoma cell line treated with vehicle for 48, 72 or 120 h.
  4. aza – OE33 esophageal adenocarcinoma cell line treated with 1 μM 5-aza-2'-deoxycytidine for 48, 72 or 120 h.
  5. Data shown for veh or aza treated OE33 are the mean ± standard deviation of triplicate reactions for each of triplicate cultures. Data shown for U ref and M ref are the mean ± standard deviation of triplicate reactions.