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Table 2 Oligonucleotide sequences representing a fragment of the CDKN2A promoter

From: Quantitation of DNA methylation by melt curve analysis

Oligonucleotide

Sequence

Unmethylated

gaagaaagaggaggggTtggTtggtTaTTagagggtggggTGgaTTGTGtgTGTtTGgTGgTtgCGgagagggggagagTaggT

Partially methylated

gaagaaagaggaggggTtggTtggtTaTTagagggtggggTGgaTTGTGtgCGTtCGgCGgTtgCGgagagggggagagTaggT

Fully methylated

gaagaaagaggaggggTtggTtggtTaTTagagggtggggCGgaTCGCGtgCGTtCGgCGgTtgCGgagagggggagagTaggT

  1. The oligonucleotide sequences representing a bisulfite modified fragment of the CDKN2A promoter that were either unmethylated, partially methylated, or fully methylated. The sequence to which the primers bind is underlined. The location of the six CpGs between the primer binding sites are in bold. Thymines which are generated following bisulfite modification and amplification are capitalised.
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BMC Cancer

ISSN: 1471-2407

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