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Table 1 Primers used in this study

From: Quantitation of DNA methylation by melt curve analysis

Gene Primer sequences 5'-3' Locationa Sizeb CpGsc
CDKN2A Forward-GAAGAAAGAGGAGGGGTTGGTTGGTTATT Reverse-ACCTACTCTCCCCCTCTCCGCAA chr9:21964847 – 21964930 84 6
TIMP3 Forward-GGYGGTATTATTTTTTATAAGGATTTG Reverse-AAACCCCRCCTCRAACTATTAAA chr22:31527488 – 31527645 158 10
MGMT Forward-IGIGTTTIGGATATGTTGGGATAGTT Reverse-ACIAAACIACCCAAACACTCACCAAA chr10:131155461 – 131155570 110 12
  1. Y – C/T
  2. R – A/G
  3. I – inosine
  4. aGenome location, as determined by UCSC Genome Browser Database (GBD, http://genome.ucsc.edu) Human March 2006 (hg18) assembly.
  5. bSize of the PCR product.
  6. cNumber of CpGs in the PCR product between the primer binding sites.