Quantitation of DNA methylation by melt curve analysis

BMC Cancer

Table 1 Primers used in this study

Gene	Primer sequences 5'-3'	Location^a	Size^b	CpGs^c
CDKN2A	Forward-GAAGAAAGAGGAGGGGTTGGTTGGTTATT Reverse-ACCTACTCTCCCCCTCTCCGCAA	chr9:21964847 – 21964930	84	6
TIMP3	Forward-GGYGGTATTATTTTTTATAAGGATTTG Reverse-AAACCCCRCCTCRAACTATTAAA	chr22:31527488 – 31527645	158	10
MGMT	Forward-IGIGTTTIGGATATGTTGGGATAGTT Reverse-ACIAAACIACCCAAACACTCACCAAA	chr10:131155461 – 131155570	110	12

Y – C/T
R – A/G
I – inosine
^aGenome location, as determined by UCSC Genome Browser Database (GBD, http://genome.ucsc.edu) Human March 2006 (hg18) assembly.
^bSize of the PCR product.
^cNumber of CpGs in the PCR product between the primer binding sites.

ISSN: 1471-2407