Figure 5
From: Quantitation of DNA methylation by melt curve analysis
Normalised melt curves of MGMT in formalin fixed paraffin embedded colorectal carcinoma tissues. DNA was isolated from whole sections of FFPE CRC tissues that had either no, clonal, or complete loss of MGMT expression as demonstrated by immunohistochemistry. Bisulfite modified DNA from CRC tissues, unmethylated reference (U ref) and methylated reference (M ref) was amplified using MGMT primers, melted and the fluorescence normalised. Data shown are the mean ± standard deviation of six replicate reactions.