Figure 4
From: Quantitation of DNA methylation by melt curve analysis
Normalised melt curves of TIMP3 in OE33 cells treated with vehicle or 5-aza-2'-deoxycytidine. Triplicate cultures of esophageal adenocarcinoma cell line OE33 were treated with either vehicle (veh) or 1 μM 5-aza-2'-deoxycytidine (aza) for 48, 72, or 120 h. Bisulfite modified DNA from treated OE33, unmethylated reference (U ref) and methylated reference (M ref) was amplified using the TIMP3 primers, melted, and the fluorescence normalised. Data shown for veh or aza treated OE33 are the mean ± standard deviation of triplicate reactions for each of triplicate cultures. Data shown for U ref and M ref are the mean ± standard deviation of triplicate reactions.