Melt curves for CDKN2A oligonucleotides. Oligonucleotide sequences representing a fragment of bisulfite modified unmethylated (U), partially methylated (P) or fully methylated (M) CDKN2A promoter (Table 2) were amplified using the CDKN2A primers and melted. The raw fluorescence data was converted to either the negative first derivative of the fluorescence with respect to temperature (-dF/dT) (A), or normalised (B), and plotted against temperature. Unmethylated oligonucleotide was spiked with either partially (U/P) or fully methylated (U/M) oligonucleotide, amplified using the CDKN2A primers and melted. Raw fluorescence data was either converted to -dF/dT (C), or normalised (D) and plotted against temperature. Data shown are the mean ± standard deviation of triplicate reactions.