Figure 8
From: Bone sialoprotein does not interact with pro-gelatinase A (MMP-2) or mediate MMP-2 activation
Effect of cell attachment to BSP on MT1-MMP-mediated activation of proMMP-2. Serum-free conditioned medium was collected from the indicated breast cancer cell lines seeded on BSP (30 μM) coated on ELISA plates, concentrated, and analyzed on zymograms. There were no significant differences in the level of proMMP-2 activation between cells grown on recombinant (r)BSP or native (n)BSP compared to cells grown on plastic.