| |
Adherent
|
Suspension
|
|---|
|
v-Src Substrates
|
Similar levela
|
More in FAK+/+
[v-Src]
|
More in FAK-/-
[v-Src]
|
Similar level
|
More in FAK+/+
[v-Src]
|
More in FAK-/-
[v-Src]
|
|---|
|
Vinculin (poY100)
| |
5 – 6
| | |
7 – 8
| |
|
p190RhoGAP (poY)*
| | |
1.5 – 2
| | |
1.5 – 2
|
|
Shc (poY239/poY240)
|
Yes
| | |
Yes
| | |
|
Sam68 (poY)*
| | |
2
|
Yes
| | |
|
PLC-γ1 (poY783)
|
Yes
| | |
Yes
| | |
|
PKC-δ (poY311)
| | |
2
|
Yes
| | |
|
Paxillin (poY118)
| | |
4
| | |
10
|
|
Paxillin (poY31)
|
Yes
| | | |
2 – 3
| |
|
Eps8 (poY)*, b
|
Yes
| | |
Yes
| | |
|
p56 Dok-2 (poY351)
| |
5
| | | |
2
|
|
Crk (poY)*
| | |
2
| | |
2
|
|
Cortactin (poY421)c
|
Yes
| | | |
2
| |
|
Connexin43 (poY265)d
| | |
2
| |
2–3
| |
|
p120Catenin (poY228)
| |
2
| | | |
2
|
|
p130 CAS (poY410)
| |
2
| | |
2
| |
|
Annexin II (poY)*
|
Yes
| | |
Yes
| |
[3]e
|
- *blotting with mAb 4G10 anti-PTyr was used to ascertain the level of tyrosine phosphorylation after immunoprecipitation with substrate-specific Abs.
-
astatistically equal level of relative substrate phosphorylation between FAK+/+[Src] and FAK-/-[Src] cells, based on triplicate experiments. Relative phosphorylation for a given experiment is calculated as the phospho- substrate signal normalized to total substrate protein level, then normalized to loading control proteins. Note that Src transformation increases GAPDH protein levels ~2-fold and decreases actin protein level ~2-fold in the FAK+/+ background in adherent cells only.
-
bfor both 97 kDa and 68 kDa Eps8 isoforms.
-
cin adherent cultures, the 80 kDa cortactin isoform protein level is reduced in the absence of FAK, but the relative phosphorylation levels are similar.
-
dconnexin-43 (poY265) levels were not normalized.
-
erelative decrease in phospho-annexin II levels in suspended FAK-/-[v-Src] cells.
