Figure 5
Phosphorylation of paxillin poY118 is required for enhanced AIG by FAK-/-[v-Src] cells. (A) Left panel- IB analysis of FAK+/+[v-Src] or FAK-/-[v-Src] cell clones ("cl.") stably transfected with empty vector (--) or a GFP-paxillinY118F-expressing vector, probed with Abs specific for GFP, paxillinpoY118 or GAPDH. Aliquots of these cells were analyzed by anchorage-independent growth (top right) or for clonogenic efficiency (bottom right) as described in Materials and Methods. Error bars, S.E. *, P < 0.01. (B) A similar analysis as in panel A except on cells stably expressing FLAG-p120Y228F, with IBs probed for FLAG, p120cateninpoY228 or GAPDH. Note that there is no statistical difference in the p120cateninY228F-mediated decrease in AIG between the FAK+/+[v-Src] and FAK-/-[v-Src] cells. (C) A similar analysis as in panel A except on cells stably expressing GST-ShcY239/240F, with IBs probed for GST-tag, ShcpoY239/240, or GAPDH.