Figure 9
Effects in mice in-vivo of local treatment with BzATP on skin apoptosis (Experiment-3). Animals (n = 5) were treated for 16 weeks either with BzATP, applied locally twice a week on the shaved dorsal skin (A, B) or with the vehicle (Control, n = 5, C, D). At the end of the experiment animals were euthanized and strips were obtained from each animal dorsal skin areas for H&E (B, D) and P2X7/TUNEL co-staining (E-L). Arrows in J show increased TUNEL staining co-localizing with P2X7 immunoreactivity in epidermal cells of the basal/parabasal layers (horizontal arrow) and of epidermal hair shaft cells (vertical arrow). Assays were repeated 3–5 times with similar trends.