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Figure 8 | BMC Cancer

Figure 8

From: Activation of P2X7-mediated apoptosis Inhibits DMBA/TPA-induced formation of skin papillomas and cancer in mice

Figure 8

Effects in mice in-vivo of local treatment with BzATP on skin apoptosis (Experiment-2). A. Mice (n = 5) were treated with BzATP, applied locally twice a week for 4 weeks on the shaved anterior skin area, and with the vehicle (Control) applied in parallel on the shaved posterior skin area. The horizontal line shows schematically separation of the anterior and posterior dorsal skin regions. At the end of the experiment animals were euthanized and skin cross sections were generated from each animal from the anterior (B, BzATP) and posterior (C, Control) dorsal skin areas for H&E (B, C), TUNEL (D-G), and DAPI (H, I) staining. D-G: TUNEL staining of BzATP-treated skin tissues (E, G) and of Control skin tissues (D, F), processed in the absence (D, E) or the presence (F, G) of the TUNEL active reagent TdT (Promega). Arrows in G show enhanced TUNEL staining in epidermal cells of the basal/parabasal layers (horizontal arrow) and of epidermal hair shaft cells (vertical arrow). H, I: DAPI (nuclear) staining. Arrows in I point to nuclei of epidermal cells in basal/parabasal regions of the epidermis at advanced stages of condensation, fragmentation and pyknosis. Data in A-I are representative of similar results in five animals.

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