Figure 6

Deletion of 5 amino acids from 214 to 218 of IGFBP5 protein promotes MDA-MB-435 breast cancer cell motility. A. Representative light-microscopy images from the migration assay. B. Quantification data of in vitro migration assay from vector transfectants (vector 1 and vector 4), wild-type IGFBP5 overexpression clones (wt-c11 and wt-c13), and mutant IGFBP5 overexpression clones (mt-c2, mt-c6, and mt-c29). The error bars represent standard deviations from 2 separate experiments with triplicate samples for each (n = 6). *, p < 0.001. C. Quantification data of in vitro migration assay from transient transfection. The error bars represent standard deviations from triplicate samples. *, p < 0.05 and **, p < 0.001.