Figure 2From: The subcellular localization of IGFBP5 affects its cell growth and migration functions in breast cancer Deletion of amino acids from 214 to 218 of IGFBP5 protein eliminates its nuclear localization. The wild-type (IGFBP5wt/pDsRed2-N1) or mutant (IGFBP5mt/pDsRed2-N1) fusion construct was transiently transfected into MBA-MD-435 breast cancer cells. The cells were fixed 48 h post-transfection and counterstained with DAPI. The images were taken by a fluorescence microscope at 63× magnification.Back to article page