Figure 2

Deletion of amino acids from 214 to 218 of IGFBP5 protein eliminates its nuclear localization. The wild-type (IGFBP5wt/pDsRed2-N1) or mutant (IGFBP5mt/pDsRed2-N1) fusion construct was transiently transfected into MBA-MD-435 breast cancer cells. The cells were fixed 48 h post-transfection and counterstained with DAPI. The images were taken by a fluorescence microscope at 63× magnification.