Figure 5

A: Before performing the cell migration and proliferation assays, protein expression of BST2 were measured by Western blot in MDA-231 cell line after transfection at 24, 36 and 48 hours with pcDNA3-BST2 (0.5 and 3 μg) and pcDNA3 empty vector (0.5 and 3 μg), respectively. Figure 5A shows that the BST2 protein expression is significantly increased by transfection with pcDNA3-BST2. β-Actin was used as a loading control. B: The graph shows that the migration activities of breast cancer cells (MDA-231) were measured at the 48-hour post-transfection with pcDNA3-BST2 (0.5 and 3 μg) and pcDNA3 empty vector (0.5 and 3 μg), respectively. Figure 5B indicates significantly increased the pcDNA3-BST2-treated cell line (MDA-231) compared to the cells treated with the empty vector (P < 0.01) (5B). C: The graph shows a FACS quantitative assessment of cells in the S phase of cell cycles in the MDA-231 cell line. Figure 5C represents the MDA-231 cell line at 24, 36 and 48-hour post-transfection with pcDNA3-BST2 (0.5 and 3 μg) and pcDNA3 empty vector (0.5 and 3 μg), respectively. The S-phase cell population significantly increased in the pcDNA3-BST2-treated cell line (MDA-231) compared to the cells treated with the empty vector (P < 0.01) (5C). These graphs show the mean values from three independent experiments.