Apoptosis ratio of MHCC97H cells increased after HSP27 RNA interference. MHCC97H cells apoptosis were detected TUNEL technology in situ in RNAi, MOCK and MHCC97H groups (A, B). In addition to TUNEL-staining, apoptotic rates were confirmed by Flow Cytometry using JC-1-staining (C). It showed the result with respect to an early indicator for apoptosis, the loss of mitochondrial membrane potential, was consistent with the result of TUNEL detection. Data are representative of 3 separate experiments. * statistical difference from control at P < 0.05.