Figure 1

Knockdown of c-MYC. c-MYC siRNA reduces c-MYC mRNA (A), protein (B) expression, and binding activity (C) as determined by quantitative RT-PCR, Western blot analysis, and by the ELISA-based TransAM-c-MYC binding activity assay (representative of two to three independent experiments). Values represent levels of c-MYC mRNA (n = 3; ± SD) (A) or protein (B) of control and c-MYC siRNA-transfected cells relative to control siRNA-transfected DAOY wt cells, which was set at 100 (A, B). Data show the mean absorbance (n = 3; ± SD) (C). * Significantly different from control values, as determined by the Student's t-test (***: P < 0.001, **: P < 0.01,*: P < 0.05).