Figure 4
From: Prodrug converting enzyme gene delivery by L. monocytogenes
PNP sectretion and prodrug conversion activity. Secretion of PNP by WL-150 pSP0-PactA-SP-PNP (a) The amount of secreted PNP was determined by Western blot analysis with PNP-specific antibodies using listerial cell lysates (CL) or supernatant proteins (SN) obtained by TCA precipitation of cell-free media after growth of WL-150 pSP0 (control) or WL-150 pSP0-PactA-SP-PNP (Pnp). (b) Prodrug-drug conversion activity of secreted PNP determined by incubation of 100 μM MePdR with the cell-free supernatants from cultures of logarithmically grown WL-150 pSP0 or WL-150 pSP0-PactA-SP-PNP at 37°C for up to 6 h. The amount of converted drug was determined by HPLC analysis.