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Table 1 Summary of the primers designed and used in the study.

From: Altered regulation of Prox1-gene-expression in liver tumors

Gene Forward primer Reverse Primer Amplicon length mRNA location
Human Prox1 (Exon 3–4) 5'-GCT CCA ATA TGC TGA AGA CC-3' 5'-ATC GTT GAT GGC TTG ACG TG-3' 120 bp 2399–2519
Human HNF4α 5'-GGG GTA CCC ATG GCC GAC TAC AGT G-3' 5'-GGG GTA CCG CGC TGA CAC CCA GGC TG-3' 140 bp 122 – 262
Human albumin 5'-CTG TGG GGC ACT TTT GAA AT-3' 5'-AAC ATC CCC TTC ACA GCA TC-3' 154 bp 698 – 851
Human CREB 5'-GAC ACC AGC TGG AGA AGG AC-3' 5'-CTT CTT GGT GTC CCA TGC TT-3' 198 bp 500 – 698
Human AP-4 5'-GTG CCC TCT TTG CAA CAT TT-3' 5'-CTG CCT TGC TGA GCT TCT CT-3' 229 bp 289 – 517
Human N-myc 5'-GAC TGT AGC CAT CCG AGG AC-3' 5'-GTT TTA ATA CCG GGG GTG CT-3' 161 bp 92 – 253
Human MAZ 5'-TAC CAC CTG AAC CGA CAC AA-3' 5'-GCT GCC TCA CAT TTC TCA CA-3' 247 bp 1040 – 1287