Figure 4

Modulation of apoptotic molecules by Ubenimex and radiation. HeLa cells were pretreated with 100 μg/ml Ubenimex for 24 h, followed by 16 Gy radiation. At 72 h after radiation treatment, whole-cell protein extracts were prepare and subjected to Western blot analysis of Bcl-xL, Bcl-2, caspase-3, cleaved caspase-3, PARP, and cleaved PARP. The β-actin protein level served as a protein loading control. The combination of Ubenimex and radiation cleaved caspase-3 and PARP, leading to apoptosis (A). Anti-apoptotic molecules, Bcl-xlL and Bcl-2, were down-regulated by Ubenimex and radiation (B).