Figure 1

TF expression and sensitivity to doxorubicin-induced apoptosis in neuroblastoma cell lines. A. western blot assay for expression of TF in two neuroblastoma cell lines SH-EP1 and SK-N-SH. Actin served as a control for equal protein loading in western blotting. B, response to doxorubicin-induced cell death of SH-EP1 and SK-N-SH. Cells were cultured with or without 10 nM FVIIa and different concentrations of doxorubicin for 48 hrs, and cell survival was determined by WST assay. Data represent mean ± SD detected in triplicate experiments. C, doxorubicin-induced apoptosis in SH-EP1 and SK-N-SH. Cells were cultured with or without 10 nM FVIIa in the absence (control) or presence of 2 μM doxorubicin for 48 hrs, and apoptotic cells were detected by annexin-V staining analyzed using flow cytometry.