Table 1 List of primers used to amplify BRCA1 exon 2, BRCA1 exon 20 and part of BRCA2 exon11.
From: Rapid detection of carriers with BRCA1 and BRCA2mutations using high resolution melting analysis
Gene | Â | Sequence |
|---|---|---|
BRCA1 | Forward primer | 5'-AAAAGATATAGATGTATGTTTTGCTAATGTGT-3' |
exon 2 | Reverse primer | 5'-TCCCAAATTAATACACTCTTGTGCTGA-3' |
BRCA1 | Forward primer | 5'-GAGTGGTGGGGTGAGATTTTTGTC-3' |
exon 20 | Reverse primer | 5'-CCTGATGGGTTGTGTTTGGTTTCT-3' |
BRCA2 | Forward primer | 5'-CGAAAATTATGGCAGGTTGTTACG-3' |
exon 11 | Reverse primer | 5'-GCTTTCCACTTGCTGTACTAAATCCA-3' |