Figure 1From: Rapid detection of carriers with BRCA1 and BRCA2mutations using high resolution melting analysis Difference plot showing different mutations relative to the wild type controls. In difference plots, the melting profile of a wild type control is chosen as a horizontal base line and the relative differences in the melting of all the other samples are plotted relative to this baseline. The figure shows difference plots for each of the 3 exons containing one of the common Ashkenazi mutations (185delAG, 5382insC and 6174delT). Each trace represents the amplicon from a different individual's DNA sample. All mutations were clearly distinct from the wild type controls. a. BRCA1 exon 2: Melt curves of each mutation (pink: 188del11, red: 185delAG and green: 185insA) were plotted against the wild type (blue). b. BRCA1 exon 20: Melt curves of each mutation (pink: 5396+1G>A, red: 5331G>A and green: 5382insC) were plotted against melt curve of the wild type (blue). c. BRCA2 exon 11: Melt curves of each mutation (pink: 6024delTA, red: 6293C>G and green: 6174delT) were plotted against melt curve of the wild type (blue).Back to article page