Actin rearrangements in C6 glioma cells treated with As
or berberine. C6 glioma cells were incubated with 5 μM As2O3 or 10 μM berberine and co-treated with 5 μM and 10 μM berberine for 24 h. Actin rearrangements were visualized by immunolocalization using anti-F-actin antibodies as described in Methods. Co-treatment with berberine and As2O3 resulted in actin (arrows) impolarization at the edges of the cell. Actin ruffing at the edges of the elongated cells was also observed and indicative of increased migration.