Figure 2
Determination of the effects of berberine-As 2 O 3 co-treatment on cell migration and growth using the scratch-wound assay. C6 glioma cells were incubated with 5 μM As2O3 or 10 μM berberine alone and with 5 μM As2O3 and 10 μM berberine for 4, 12, and 24 h and the migration was visualized as described in Methods (A). The percentage of surface area filled by the C6 cells was subsequently quantified by densitometric analyses relative to that of the control which was set at 100% as shown in the graph (B). Data are presented as means ± SD based on three independent experiments. * and ** indicate means that are significantly different when compared to the control group of 12 h incubation with P < 0.05 and P < 0.01, respectively. # and ## indicate means that are significantly different when compared to the control group of 24 h incubation with P < 0.05 and P < 0.01, respectively.