Figure 1
Evaluation of the cell viability of C6 glioma and human U-87 cells treated with berberine or As 2 O 3 , and co-treated with As 2 O 3 and berberine for 24 h. C6 glioma cells (A) and human U-87 cells (B) were incubated with 1, 2, 5, 10, and 20 μM berberine or As2O3 for 24 h and the proportion of surviving cells was determined using the MTT assay as described in Methods. (C) C6 glioma and human U-87 cells were co-treated with 5 μM As2O3 and 1, 2, 5, 10, and 20 μM berberine for 24 h, and the cell growth was determined as described in Methods. Control cells were treated with As2O3 alone. The experiments were performed in triplicate and data are presented as means ± SD. * and ** indicate means that are significantly different when compared to the control group of C6 with P < 0.05 and P < 0.01, respectively. # and ## indicate means that are significantly different when compared to the control group of U-87 with P < 0.05 and P < 0.01, respectively.