K-rasmutations in sinonasal cancers in relation to wood dust exposure

BMC Cancer

Table 3 Reaction conditions for all the PCR reactions.

Assay\PCR conditions	KCl	Tris-HCl	MgCl₂	dNTP	Taq polymerase	Primers _{forward + reverse}	pH
RFLP12	50 mM	20 nM	0.25 mM	0.2 mM	0.75 unit	0.5 μM	8.4
RFLP13	50 mM	20 nM	0.25 mM	0.2 mM	0.75 unit	0.5 μM	8.4
RFLP61	50 mM	10 nM	1.5 mM	0.2 mM	1.0 unit	1.0 μM	8.3
SEQ1213	50 mM	10 nM	1.5 mM	0.2 mM	1.0 unit	1.0 μM	9.0
SEQ61	50 mM	10 nM	1.5 mM	0.2 mM	1.0 unit	1.0 μM	9.0

ISSN: 1471-2407