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Table 3 Reaction conditions for all the PCR reactions.

From: K-rasmutations in sinonasal cancers in relation to wood dust exposure

Assay\PCR conditions KCl Tris-HCl MgCl2 dNTP Taq polymerase Primers forward + reverse pH
RFLP12 50 mM 20 nM 0.25 mM 0.2 mM 0.75 unit 0.5 μM 8.4
RFLP13 50 mM 20 nM 0.25 mM 0.2 mM 0.75 unit 0.5 μM 8.4
RFLP61 50 mM 10 nM 1.5 mM 0.2 mM 1.0 unit 1.0 μM 8.3
SEQ1213 50 mM 10 nM 1.5 mM 0.2 mM 1.0 unit 1.0 μM 9.0
SEQ61 50 mM 10 nM 1.5 mM 0.2 mM 1.0 unit 1.0 μM 9.0