Table 1 Primers and PCR conditions used for MDM4 screening. Summary of primers, annealing temperatures and PCR product sizes for the eleven exons of the MDM4 gene. Forward and reverse primer sequences are listed. Exons 2 – 11 are coding exons. Exon 11 was amplified in two overlapping parts in order to make it more accessible to sequencing analysis.
From: Mutation analysis of the MDM4gene in German breast cancer patients
Exon | Primer | Annealing (°C) | Product size (bp) |
|---|---|---|---|
1 | 5'-TCTGATCTCCTAATACACGTCTG-3' 5'-GCCTGCTCCTCACTCTCCAG-3' | 62 | 416 |
2 | 5'-CTGGTTGCCTTTGTGTGAATG-3' 5'-CTGGGATTACAGTCATGAGAC-3' | 62 | 296 |
3 | 5'-AGAGGTTCTCTTGTTCCATAG-3' 5'-GATCTGAGATCGCACCACTG-3' | 62 | 253 |
4 | 5'-CAGATCAGTTCATTTCTGTGCTG-3' 5'-CATATTCTCAGTGCCTCATAGG-3' | 62 | 520 |
5 | 5'-GATTCTGCCTTTGTATGCCTTAC-3' 5'-CTCAAAGCTGTGATACAGACTG-3' | 62 | 234 |
6 | 5'-CAGCCAACATGGAGAAGTAC-3' 5'-CAGAGAAGGTTCACTCTGTC-3' | 62 | 356 |
7 | 5'-GGTGAGCCAGAATGGAACTG-3' 5'-ACAATGGTAGTAACTAGGCTG-3' | 62 | 334 |
8 | 5'-AGCTCTGCCACTAAGACAG-3' 5'-CAAGAGTAACCAAGAATGTTACC-3' | 62 | 389 |
9 | 5'-GATGTAGTGTGACGACATTGAG-3' 5'-CAACAGATGATACTATGAGCAC-3' | 62 | 311 |
10 | 5'-CTACTACTGAAATGCCAACTAGAAG-3' 5'-GGATTACATCATCTGAAGATGG-3' | 62 | 329 |
11 (a) | 5'-GCTTAGTGAGAGGATGTGAATG-3' 5'-CTGTCGTTAGACCTAAAGATGC-3' | 59 | 431 |
11 (b) | 5'-CTGTCGTTAGACCTAAAGATGC-3' 5'-CCTAAGAACATTCTCTGACAG-3' | 59 | 475 |