Table 1 Characteristics of the studied polymorphisms with primer sequences and restriction enzymes
Gene | Primer sequences | Restriction enzyme | Polymorphism |
|---|---|---|---|
GSTP1 | [F]:G5'-GTAGTTTGCCCAAGGTCAAG-3' [R]:G5'-AGCCACCTGAGGGGTAAG-3' | BsmAI | Ile105Val (313A>G) |
ABCB1 | [F]:G5'-GATCTGTGAACTCTTGTTTTC-3' [R]:G5'-GAAGAGAGACTTACATTAGGC-3' | DpnII | Ile1145Ile (3435C>T) |
MTHFR | [F]:G5'-CTTGAACAGGTGGAGGCCAGC-3' [R]:G5'-AGGACGGTGCGGTGAGAGTG-3' | HinfI | Ala222Val (677C>T) |
TYMS | [F]:G5'-GACCCCGCCGAGCAGGAAGA-3' [R]:G5'-GTGCCCGTGCGGTCGTCCTT-3' | - | 28-bp repeat (5'-UTR) |
ERCC2 | [F]:G5'-TCTGCAGGAGGATCAGCTG-3' [R]:G5'-GCAAGACTCAGGAGTCAC-3' | PstI | Lys751Gln (2251A>C) |
XRCC1 | [F]:G5'-TTGTGCTTTCTCTGTGTCCA-3' [R]:G5'-TCCTCCAGCCTTTTCTGATA-3' | MspI | Arg399Gln (1196G>A) |