Cytoplasmic protein activity levels. (A) Cytoplasmic activated protein profiles are represented by a grayscale map of IHC intensity levels. The two largest branches derived from an unsupervised hierarchical clustering analysis show similarities in tumor specimens (a) and histologically normal ducts (b). (B) Representative IHC images of activated mTOR (i, ii) and ERK (iii, iv) from the TMA of tumor (T-B4/A4, i, iii) and ducts of non-PDAC pancreas (D-J3, ii, iv, arrows). A typical duct is digitally enlarged (insert).