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Table 1 Clinicopathological parameters and mRNA expression in the patients (n = 64)

From: mRNA expression of the DNA replication-initiation proteins in epithelial dysplasia and squamous cell carcinoma of the tongue

Characteristic

No.(%)

CDC6

CDT1

MCM2

CDC45

Age (years)

Median 54 (range 17–82)

     

Sex

     

Male

33(52%)

2.17E-03 ± 8.24E-04

1.10 ± 0.44

1.20 ± 0.24

2.17 ± 0.41

Female

31(48%)

6.28E-03 ± 1.97E-03

1.04 ± 0.34

1.28 ± 0.29

2.67 ± 0.89

Epithelial dysplasia (precancerous, n = 31)

Mild

8(26%)

4.68E-04 ± 1.42E-04

0.64 ± 0.14

0.19 ± 0.05

0.35 ± 0.72

Moderate

8(26%)

6.59E-03 ± 3.20E-03

0.99 ± 0.20

1.03 ± 0.18

2.29 ± 0.85

Severe

15(48%)

8.28E-03 ± 3.40E-03

1.94 ± 0.36

2.48 ± 0.35

3.32 ± 0.54

Histological grade (SCC, n = 33)

G1

13(40%)

6.87E-03 ± 2.67E-03

0.92 ± 0.19

1.15 ± 0.22

2.48 ± 0.82

G2

10(30%)

7.87E-03 ± 6.51E-04

0.72 ± 0.17

0.99 ± 0.30

1.97 ± 0.59

G3

10(30%)

8.61E-03 ± 1.30E-03

1.40 ± 0.30

1.73 ± 0.73

2.45 ± 0.13

T-stage

T1

10(34%)

7.61E-03 ± 4.45E-03

1.01 ± 0.27

0.71 ± 0.20

3.17 ± 0.93

T2

19(66%)

5.02E-03 ± 7.62E-04

0.94 ± 0.14

1.56 ± 0.42

1.57 ± 0.44

Lymph node metastases

N0

12(41%)

6.11E-04 ± 1.66E-04

0.74 ± 0.16

0.60 ± 0.15

1.27 ± 0.35

N1

11(38%)

3.50E-03 ± 1.02E-03

0.90 ± 0.15

1.03 ± 0.64

2.00 ± 0.52

N2

6(21%)

1.02E-02 ± 7.03E-03

1.28 ± 0.28

2.00 ± 0.42

3.54 ± 0.15

Clinical stage

I

4(13%)

4.21E-04 ± 3.08E-04

0.89 ± 0.14

0.15 ± 0.04

1.13 ± 0.36

II

8(28%)

8.02E-04 ± 1.13E-04

0.74 ± 0.16

0.85 ± 0.21

1.55 ± 0.46

III

11(38%)

3.50E-03 ± 1.02E-03

1.07 ± 0.28

1.03 ± 0.64

2.01 ± 0.52

IV

6(21%)

1.02E-02 ± 7.03E-03

1.78 ± 0.82

2.00 ± 0.42

3.54 ± 0.15

  1. T-stage (tumor size): Tl, < 2 cm; T2, 2 to 4 cm; T3 > 4 cm
  2. G1, highly differentiated; G2, moderately differentiated; G3, poorly differentiated
  3. Stage I, T1N0M0; stage II, T2N0M0; stage III, T3N0M0, T1-3N1-2M0; stage IV, T4N0-3M0
  4. Shown is quantitative gene expression analysis by QRT-PCR in different formalin-fixed, paraffin-embedded specimens. Levels of CDC6, CDT1, MCM2 and CDC45 mRNA were determined by QRT-PCR and all measurements are shown relative to the expression levels of the β-actin housekeeping gene. Shown are the mean normalized values of three independent experiments, each in duplicates, using different cDNA samples.