Figure 3

Box plot presentation of the relative RNA levels of H1x and H1.0 determined by quantitative real-time RT-PCR. Total RNA was extracted from normal lung tissues (Table 1, samples no. 1 and 3), NETs from lung (no. 2 and 4), normal tissues from small intestine (no. 8 and 9), NETs from small intestine (no. 10, 11 and 12), normal tissue from pancreas (no. 15) and pancreatic NETs (no. 17, 18 and 19) and used for reverse transcription. The generated cDNA from each patient was used for triplicate quantitative real-time PCR. Gene expression levels of H1x and H1.0 were determined by relative quantification using the expression of histone H1.2 for normalization. Wilcoxon test was used for statistical analysis. The mRNA level of H1x was significantly increased in NET tissue of lung (p = 0.004) and small intestine (p = 0.01). Horizontal line, median; inner box, 25 and 75% interval; outer spread, minimum and maximum.