Figure 2

(A) Relative protein amounts of the H1 histone subtypes H1x, H1.2 and H1.0 in non-neoplastic and neuroendocrine tumour tissues. Determination of relative protein levels of histone H1x, main type subtype H1.2 and replacement histone H1.0 was done by Western blot analysis. Lane 1, normal tissue of lung (Table 1, sample no. 3), lane 2, neuroendocrine carcinoid of lung (no. 2), lane 3, poorly differentiated neuroendocrine small cell carcinoma of lung (no. 4), lane 4, normal tissue of lung (no. 5), lane 5, neuroendocrine carcinoid of lung (no. 6), lane 6, poorly differentiated small cell carcinoma of lung (no. 7), and lanes 7 and 8, normal tissue of small intestine (no. 8 and 9), lane 9, well-differentiated neuroendocrine carcinoma of small intestine (no. 10), lane 10, poorly differentiated neuroendocrine carcinoma (no. 11), and lane 11 well-differentiated neuroendocrine carcinoma of small intestine (no. 12), lane 12, normal tissue of small intestine (no. 8), lanes 13 and 14, neuroendocrine carcinoid of small intestine (no. 13 and 14), lane 15 and 16, normal tissue of pancreas (no. 15 and 16), lane 17, pancreatic neuroendocrine tumour of poor differentiation (no. 18) and lane 18, of good differentiation (no. 19), lane 19, normal tissue of pancreas (no. 16), lanes 20 and 21, well-differentiated carcinoma of pancreas (no. 17 and 20), lane 22, normal tissue of liver and lane 23, liver metastasis of well-differentiated carcinoma of pancreas (no. 21 and 22). (B) Densitometric quantitation of the immunoblots shown in (A). Ratios of H1x and H1.0 to H1.2 were calculated and standard deviations are indicated.